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Competent Cells

Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.

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1

Invitrogen™ MAX Efficiency™ DH5αF'IQ Competent Cells

MAX Efficiency DH5αF'IQ Competent Cells are high transformation efficiency chemically competent cells prepared by a patented modification of the procedure of Hanahan. This strain is a derivative of the DH5α strain, sharing the same benefits.

2

Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli

MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
High-throughput Compatibility High
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Product Type Chemically Competent Cells
Product Line MultiShot™
Contains F' Episome No
3

Invitrogen™ One Shot™ BL21(DE3)pLysS Chemically Competent E. coli

One Shot BL21(DE3)pLysS Chemically Competent E. coli cells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET.

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Shipping Condition Dry Ice
Blue/White Screening No
Cloning Methylated DNA No
Transformation Efficiency Level Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
ToxicProteins No
High-throughput Compatibility Low
Improves Plasmid Quality No
ImprovesProteinStability Yes (lon, ompT)
Promoter T7
Preparing Unmethylated DNA Yes (dcm)
Reduces Recombination No
ImprovesRNAStability Yes (pLysS)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Chloramphenicol)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (B)
Product Line One Shot™
Contains F' Episome No
4

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot™
Contains F' Episome No
5

Invitrogen™ Tali™ Cell Cycle Kit

Convenient and accurate determination of the percentage of cells in each phase of the cell cycle

6

Gibco™ Expi293™ Met (–) Expression Medium

Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.

7

Invitrogen™ ElectroMAX™ DH12S Cells

ElectroMAX DH12S Cells, a derivative of DH10B cells, are suitable for transformation by electroporation. They may be used for production of single-stranded (ss)DNA and in procedures requiring high transformation efficiencies, such as generation of cDNA, genomic, and subtractive cDNA libraries.