Thermo Scientific™ Acclaim™ PepMap™ 100 C18 HPLC Columns

Use for high-resolution tryptic, natural, and synthetic peptide analyses. Its high loading capacity is exceptional for low abundant peptides in complex proteomic samples.

Product Code	Brand	Length (Metric)	Diameter (Metric)	Particle Size	Type
11342263	Thermo Scientific™ 164567	50 mm	0.075 mm	3 μm	Analytical Column, NanoViper
11352263	Thermo Scientific™ 164568	150 mm	0.075 mm	3 μm	Analytical Column
11362263	Thermo Scientific™ 164569	250 mm	0.075 mm	3 μm	Analytical Column
11372263	Thermo Scientific™ 164570	500 mm	0.075 mm	3 μm	Analytical Column
11302273	Thermo Scientific™ 164572	150 mm	1 mm	3 μm	Analytical Column
11312113	Thermo Scientific™ 160434	5 mm	1 mm	5 μm	Trap Cartridge
11362113	Thermo Scientific™ 160454	5 mm	0.3 mm	5 μm	Trap Cartridge
11322113	Thermo Scientific™ 160438	15 mm	1 mm	5 μm	Trap Cartridge
11382243	Thermo Scientific™ 164197	150 mm	0.1 mm	5 μm	Trap Column
11332253	Thermo Scientific™ 164213	150 mm	0.2 mm	5 μm	Trap Column
11392243	Thermo Scientific™ 164199	150 mm	0.1 mm	5 μm	Trap Column
11352103	Thermo Scientific™ 160323	150 mm	0.075 mm	5 μm	Analytical Column
11362103	Thermo Scientific™ 160326	250 mm	0.075 mm	5 μm	Analytical Column
11352023	Thermo Scientific™ 164562	150 mm	0.05 mm	2 μm	Analytical Column, NanoViper
11372013	Thermo Scientific™ 164537	150 mm	0.3 mm	2 μm	Analytical Column
11392253	Thermo Scientific™ 164563	50 mm	0.75 mm	2 μm	Analytical Column, NanoViper
11382013	Thermo Scientific™ 164540	500 mm	0.75 mm	2 μm	Analytical Column
11342013	Thermo Scientific™ 164534	150 mm	0.075 mm	2 μm	Analytical Column
11342023	Thermo Scientific™ 164561	50 mm	0.05 mm	2 μm	Analytical Column, NanoViper
11332023	Thermo Scientific™ 164560	50 mm	0.3 mm	2 μm	Analytical Column
15597520	Thermo Scientific™ 164941	250 mm	0.75 mm	2 μm	Analytical Column
11352013	Thermo Scientific™ 164535	150 mm	0.075 mm	3 μm	Trap Column
15810705	Thermo Scientific™ 164738	500 mm	0.075 mm	2 μm	Analytical Column
15820705	Thermo Scientific™ 164739	500 mm	0.075 mm	3 μm	Analytical Column
15567530	Thermo Scientific™ 164942	500 mm	0.075 mm	2 μm	Analytical Column
15572920	Thermo Scientific™ 164939	750 mm	0.075 mm	2 μm	Analytical nano Column
15800705	Thermo Scientific™ 164454	50 mm	1 mm	2 μm	Analytical Column, NanoViper
15588090	Thermo Scientific™ 164946	20 mm	75 μm	3 μm	Trap Column
12739835	Thermo Scientific™ 164704	150 mm	0.05 mm	2 μm	Analytical Column
15527660	Thermo Scientific™ 164943	150 mm	0.05 mm	2 μm	Analytical Column
11382253	Thermo Scientific™ 164261	250 mm	0.075 mm	3 μm	Analytical Column
11342103	Thermo Scientific™ 160321	150 mm	0.075 mm	3 μm	Analytical Column
11312263	Thermo Scientific™ 164564	150 mm	0.1 mm	5 μm	Trap Column
15517460	Thermo Scientific™ 164940	150 mm	0.075 mm	2 μm	Analytical Column
17294434	Thermo Scientific™ 164750	150 mm	0.1 mm	5 μm	Trap Column
17204444	Thermo Scientific™ 164751	150 mm	0.3 mm	3 μm	Analytical Column, Nanoviper
13486836	Thermo Scientific™ 164711	150 mm	1 mm	2 μm	Analytical Column
15518080	Thermo Scientific™ 164944	250 mm	0.05 mm	2 μm	Column
15588080	Thermo Scientific™ 164945	500 mm	0.05 mm	2 μm	Column

Description

Feature high loading capacity for exceptional suitability in the analysis of low abundant peptides in complex proteomics samples with Thermo Scientific™ PepMap™ 100 C18 LC Columns. PepMap LC columns offer lengths targeting fast or high resolution peptide separation.

• High resolution in protein identification, biomarker discovery, and systems biology
• Particular loadability allows highest sensitivity in LC/MS
• Designed for TFA-free LC-MS, minimizing ion-suppression effects
• Optimized for coupling to ESI/MS and MALDI-MS
• Highest column-to-column reproducibility
• Easy-to-use, cutting-edge miniaturized HPLC
• Preassembled with nanoViper column inlet fittings for easy column installation

Key advantages of nanoViper fittings

• Ease of use, no sleeve for small capillaries
• Tool-free assembly
• No column damage from overtightening: capable of withstanding pressures up to 1000 bars and is compatible with third-party valves and unions
• No experimental failure due to bad connections

Ideal for high-resolution peptide mapping of tryptic, natural, and synthetic peptides as well as LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology.

• High loading capacity
• Use for the analysis of low abundant peptides often found in complex proteomics samples
• Select the column length and I.D. for your throughput, sensitivity, and amount of sample available requirements
• Choose from 50, 150, 250, 500mm lengths and 50, 75, 300 and 1000μm I.D.
• Superior protein and peptide separations
• Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
• Outstanding separation efficiency
• Superior resolution and recovery
• Easy installation with nanoViper™ Fingertight Fittings

PepMap nanocolumns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.

• Requires no tools
• Provides virtually zero-dead-volume connections
• Withstands pressures up to a 1000 bar

About Nano, capillary, and micro Acclaim PepMap columns

Nano (75μm), capillary (300μm) and micro (1000μm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and microcolumns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and microcolumn in a variety of chemistries for peptides and protein analysis.

Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one-dimensional peptide mapping experiments and 2D-LC analyses.

Specifications

• Pore Size: 100 Å
• USP Type: L1
• Stationary Phase: C18
• Phase: Reversed Phase

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.