Presenilin 1 Monoclonal Antibody (APS 11), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA1751

Product Code. 11575282

Description

MA1-751 detects presenilin 1 protein (PS1) from mouse, rat, human, and nonhuman primate samples. No cross-reactivity is seen with presenilin 2. MA1-751 has successfully been used immunofluorescence, immunocytochemistry, immunohistochemistry, Western blot , and ELISA procedures. By Western blot, this antibody detects an ∽28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1. In formalin-fixed, paraffin embedded sections of human brain, this antibody showed strong staining of both the plaque core and dystrophic neurites. The MA1-751 immunogen is a synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer's disease. In addition to PEN2, nicastrin and APH-1, Presenilin1 forms the -gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-alpha-converting enzyme (TACE) and the beta-site cleavage enzyme (BACE) protease families, -gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and -gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and -gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Multiple isoforms of presenilin1 are known to exist.

Specifications

• Antigen: Presenilin 1
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: P49768, P49769, P97887
• Gene Symbols: PSEN1
• Immunogen: Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Non-human Primate, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (PFA fixed), Immunoprecipitation, Western Blot
• Clone: APS 11
• Conjugate: Unconjugated
• Gene: PSEN1
• Gene Alias: AD3; Ad3h; Alzheimer Disease 3; FAD; Minilin; presenilin 1; presenilin 1 (Alzheimer disease 3); presenilin1; presenilin-1; Presenilin-1 CTF subunit; Presenilin-1 CTF12; Presenilin-1 NTF subunit; Protein S182; PS1; PS-1; PS1-CTF12; PSEN1; Psnl1; S182; S182 protein; Senilin 1
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 19164, 29192, 5663
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid