Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Brand:  Applied Biosystems™ 4398893

Product Code. 10279094

Includes: AmpliTaq 360 DNA Polymerase is supplied at 5U/μL with 10X AmpliTaq 360 PCR Buffer, 25mM M2Cl₂, and 360 GC Enhancer. Typically, 1.5mL of each of AmpliTaq 360 Buffer, 25mM MgCl₂, and 360 GC Enhancer is supplied with each 250U of enzyme. Storage Buffer Enzyme is supplied in 20mM Tris, pH 9.0, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% (w/v) Tween 20, 50% (v/v) glycerol. Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli. All solutions are prepared in deionized, filter-sterilized water.

Description

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. Compared to the original AmpliTaq DNA Polymerase, AmpliTaq 360 DNA Polymerase is purified by an additional proprietary separation process that reduces contaminating bacterial DNA sequences from the enzyme preparation. This ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and, when combined with the proprietary AmpliTaq 360 Buffer Kit, promotes the amplification of a variety of templates, including those from bacterial and human genomes.

Features

• Optimized for the broadest range of targets, from everyday to challenging
• Unmatched sensitivity and yield
• Market-leading GC enhancer for robust amplification of GC-rich sequences
• Achieves the highest quality sequencing data

Notes

• DreamTaq DNA Polymerase is recommended for superior PCR performance.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Content And Storage:

  • 10X AmpliTaq 360 PCR Buffer, 1.5 mL for each 250 U of enzyme
  • 25 mM MgCl₂, 1.5 mL for each 250 U of enzyme
  • 360 GC Enhancer, 1.5 mL for each 250 U of enzyme
  
  Storage Buffer Enzyme is supplied in 20 mM Tris (pH 9.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% (w/v), Tween 20, 50% (v/v) glycerol
  
  Source: Recombinant, modified form of the Thermus aquaticus DNA polymerase gene expressed in E. coli

  All solutions are prepared in deionized, filter-sterilized water.
  
  When stored at -15°C to -25°C, this product maintains performance at least until the expiration date on the tube label. Before use, thaw frozen components to room temperature and mix well.
  Product is guaranteed up to 12 months as indicated by the expiration date.

• GC-Rich PCR Performance: High
• Exonuclease Activity: 5' - 3'
• Concentration: 5 U/μL
• Overhang: 3'-A
• Size (Final Product): 5 kb or less

• Format: Stand-alone enzyme
• Polymerase: AmpliTaq Gold 360 DNA Polymerase
• Quantity: 3000 Units
• Fidelity (vs. Taq): 1X
• Reaction Format: Separate Components
• Starting Material: DNA

For Research Use Only. Not for use in diagnostic procedures.