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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Brand: Applied Biosystems™ 4304134
3003.00 GBP valid until 2024-12-31
Use promo code "21651" to get your promotional price.
Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)
Description
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and polymerase chain reaction amplification of a specific target RNA from either total RNA of mRNA. The reagents in this kit facilitate two-step RT-PCR.
Two-step RT-PCR is performed in two separate reactions, useful when detecting multiple transcripts from a single cDNA reaction, or when storing a portion of the cDNA.
Advantages of TaqMan Reverse Transcription Reagents
• Contains MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay setup
• Compatible with two-step RT-PCR reactions
Using TaqMan Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
Specifications
Includes 10 units of Cat. No. N8080234. Each unit contains: |
|
Reverse Transcription | |
2,000 reactions | |
Yes | |
Real Time PCR (qPCR) | |
First-Strand cDNA | |
Separate components | |
Up to 5 kb |
Kit | |
42°C | |
MultiScribe | |
Dry Ice | |
50 U/μL | |
2,000 Reactions | |
Reverse Transcription | |
RNA |
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For Research Use Only. Not for use in diagnostic procedures.