Thermo Scientific™ BacMam Akt [pSer473] Cellular Assay Kit

The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.

Brand:  Thermo Scientific™ A12899

Product Code. 10776041

Description

The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT. BacMam provides a convenient genetic delivery tool for a GFP-AKT fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-AKT at Ser473.

Specifications

• Assay Entry: Cell-based terbium modification activity (transient), Cell-based terbium phospho-specific activity (transient)
• Content And Storage: Kit contains Terbium-labeled anti-AKT antibody, BacMam AKT Virus, and 6X Lysis Buffer. Store as indicated in handling documentation.
• Detection Method: Fluorescence
• Human Protein Class: Kinases, Cell Signalling
• Product Line: LanthaScreen™
• Readout: End Point
• Target Entry: AKT1
• System Type: LanthaScreen™

• Cell State: Cells Not Included
• Description: BacMam Akt [pSer473] Cellular Assay Kit, 1 Kit, Fluorescent Detection Method, Kinases Druggable Target, TR-FRET Technique, LanthaScreen™ System, End Point Readout, GFP (EmGFP) Reporter
• For Use With (Application): TR-FRET
• Includes: Terbium-labeled anti-AKT antibody, BacMam AKT virus and 6X lysis buffer
• Quantity: 1 kit
• Reporter Gene: GFP (EmGFP)
• Type: Cellular Assay Kit
• Druggable Target: Kinases

For Research Use Only. Not for use in diagnostic procedures.