General Cell Based Assays
General Cell Based Assays
Thermo Scientific™ LanthaScreen™ STAT3 GripTite™ Cell Line
The JAK/STAT3 signaling pathway is known to be activated by cytokines such as IL-6. In this pathway, binding of IL-6 to its cell-surface receptors results in the activation of JAKs, which in turn phosphoactivate STAT3 proteins at a specific tyrosine residue (Y705).
Thermo Scientific™ Tango™ EDG7-bla U2OS Cells
Tango™ EDG7-bla U2OS cells contain the human Endothelial Differentiation Gene 7 (EDG7) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Thermo Scientific™ Kinase Tracer 236, 50 μM
In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 236 to a kinase is detected by addition of a Eu-labeled anti-tag antibody.
Thermo Scientific™ Tango™ CCR1-bla U2OS Cells
The Tango™ CCR1 -bla U2OS cells contain the human Chemokine (C-C Motif) Receptor 1 (CCR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Thermo Scientific™ LanthaScreen™ STAT5 (JAK2 V617F) U2OS Cell Line
LanthaScreen™ GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context.
Thermo Scientific™ GeneBLAzer™ RXRα-UAS-bla HEK 293T Cells
RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UASbla HEK 293T cell line.
Thermo Scientific™ GeneBLAzer™ GPR24-Gqi5-NFAT-bla CHO-K1 Cells
GeneBLAzer™ GPR24-Gqi5-NFAT-bla CHO-K1 cells contain the human Melanin-concetrating hormone receptor (GPR24) stably integrated into the GeneBLAzer™ Gqi5-NFAT-bla CHO-K1 cell line.
Thermo Scientific™ BacMam Akt [pSer473] Cellular Assay Kit
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.
Thermo Scientific™ LanthaScreen™ STAT1 U2OS Cell Line
The JAK/STAT1 signaling pathway is known to be activated by type I/II interferons such as interferon-gamma and interferon-alpha.
Thermo Scientific™ GeneBLAzer™ EDG3-Gα15 HEK 293T DA Assay Kit
GeneBLAzer™ EDG3-Gα15 HEK 293T DA (Division Arrested) cells and EDG3-Gα15-NFAT-bla HEK 293T cells contain the human EDG3 receptor stably integrated in the GeneBLAzer™ Gα15-NFAT-bla HEK 293T cell line.
Thermo Scientific™ GeneBLAzer™ M3-NFAT-bla CHO-K1 Cells
GeneBLAzer™ M3-NFAT-bla CHO-K1 cells contain the human Acetylcholine (Muscarinic) Receptor 3 (M3) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.
Thermo Scientific™ BacMam Akt [pThr308] Cellular Assay Kit
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.
Thermo Scientific™ Tango™ SSTR5-bla U2OS DA Assay Kit
The Tango™ SSTR5-bla U2OS cells contain the human Somatostatin Receptor 5 (SSTR5) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.
Thermo Scientific™ LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit
The LanthaScreen™ TR-FRET ER alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential estrogen receptor (ER) alpha ligands as agonists or antagonists of ligand-dependent coactivator recruitment.
Thermo Scientific™ PolarScreen™ Progesterone Receptor Competitor Assay Kit, Red
The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Red, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands.