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Thermo Scientific™ Tango™ ADORA1-bla U2OS Cells

Product Code. 10467118
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Quantity:
2 x 106 Cells
Unit Size:
Each
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Product Code. 10467118

Brand: Thermo Scientific™ K1445

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Description

The Tango™ ADORA1-bla U2OS cells contain the human Adenosine A1 receptor (ADORA1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line.

The Tango™ ADORA1-bla U2OS cells contain the human Adenosine A1 receptor (ADORA1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin/TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ ADORA1-bla U2OS cells have been functionally validated for Z' factor and EC50 concentrations of NECA. In addition, Tango™ ADORA1-bla U2OS cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000674.1
Assay Entry Cell-based beta-arrestin recruitment
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• Tango™ ADORA1-bla U2OS cells
• Tango™ ADORA1-bla U2OS Assay Protocol
• Certificate of Analysis

The Tango™ ADORA1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cytoplasmic Tail Tail from AVPR2 GPCR
Detection Method Fluorescence
For Use With (Application) GPCRs
Gene Symbol ADORA1A
Includes ADORA1-bla U2OS cells, ADORA1-bla U2OS Assay Protocol, Certificate of Analysis
Population Status Pool of Stable Cells
Product Line Tango
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry ADORA1
Type Dividing Cells
Cell Line U2OS
System Type Tango™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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