Thermo Scientific™ CellSensor™ ISRE-bla HEK293T Cell Line

The CellSensor™ ISRE-bla HEK 293T cell line is responsive to interferon alpha and can be used to probe the JAK/STAT signaling pathway.

Brand:  Thermo Scientific™ K1655

Product Code. 10563084

Description

Interferon cell signaling is a critical pathway involved in viral defense and autoimmune disease. The CellSensor™ ISRE-bla HEK 293T cell line is responsive to interferon alpha and can be used to probe the JAK/STAT signaling pathway. The CellSensor™ ISRE-bla HEK 293T cell line contains the β-lactamase reporter gene under the control of the interferon stimulated response element (ISRE). This cell line was engineered by transduction of the ISRE-bla construct into HEK 293T cells by lentivirus. Flow cytometry was then used to isolate a pool of clones responsive to interferon alpha a stimulation. A dose response curve for this assay against interferon alpha is available in the validation packet. Academic and non-profit customers, please inquire for special pricing.

Specifications

• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
• Product Line: CellSensor™
• Target Entry: JAK/STAT (Type I IFN) pathway, TYK2, JAK1
• System Type: CellSensor™

• Cell State: Dividing Cells
• For Use With (Application): Cytokines, Signaling Pathway
• Quantity: 1 Vial
• Type: Dividing Cells
• Druggable Target: Signaling Pathway

For Research Use Only. Not for use in diagnostic procedures.