Thermo Scientific™ GeneBLAzer™ M3-NFAT-bla CHO-K1 Cells

GeneBLAzer™ M3-NFAT-bla CHO-K1 cells contain the human Acetylcholine (Muscarinic) Receptor 3 (M3) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

Brand:  Thermo Scientific™ K1716

Product Code. 10773734

Description

GeneBLAzer™ M3-NFAT-bla CHO-K1 cells contain the human Acetylcholine (Muscarinic) Receptor 3 (M3) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1534) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer™ M3-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC₅₀ concentrations of Carbachol. In addition, GeneBLAzer™ M3-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The M3 receptor is localized in smooth muscle, exocrine glands, and the central nervous system. M3 has been implicated in autoimmune and neurological disorders.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

Specifications

• Accession Number: NM_000740.2
• Cell State: Dividing Cells
• Detection Method: Fluorescence
• Gene Symbol: M3
• Population Status: Single Cell Clone Origin
• Quantity: 2 x 10⁶ Cells
• Target Entry: M3
• Cell Line: CHO-K1
• Druggable Target: GPCR

• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.
  
  Includes:
  • GeneBLAzer™ M3-NFAT-bla CHO-K1 cells
  • GeneBLAzer™ M3-NFAT-bla CHO-K1 Assay Protocol
  • Certificate of Analysis
  
  The GeneBLAzer™ M3-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• For Use With (Application): FRET
• Includes: M3-NFAT-bla CHO-K1 cells, M3-NFAT-bla CHO-K1 Assay Protocol, Certificate of Analysis
• Product Line: GeneBLAzer™
• Reporter Gene: BLA (Beta-Lactamase)
• Type: Dividing Cells
• System Type: GeneBLAzer™

For Research Use Only. Not for use in diagnostic procedures.