Thermo Scientific™ BacMam Akt [pThr308] Cellular Assay Kit

The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.

Brand:  Thermo Scientific™ A12900

Product Code. 10786041

Description

The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT. BacMam provides a convenient genetic delivery tool for a GFP-AKT fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-AKT at Thr308.

Specifications

• Assay Entry: Cell-based terbium modification activity (transient), Cell-based terbium phospho-specific activity (transient)
• Content And Storage: Kit contains Terbium-labeled anti-AKT antibody, BacMam AKT Virus, and 6X Lysis Buffer. Store as indicated in handling documentation.
• For Use With (Application): TR-FRET
• Includes: Cells not includes
• Quantity: 1 kit
• Reporter Gene: GFP (EmGFP)
• System Type: LanthaScreen™

• Cell State: Cells Not Included
• Detection Method: Fluorescence
• Human Protein Class: Kinases, Cell Signalling
• Product Line: LanthaScreen™
• Readout: End Point
• Target Entry: AKT1
• Druggable Target: Kinases

For Research Use Only. Not for use in diagnostic procedures.