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Thermo Scientific™ BacMam Akt [pThr308] Cellular Assay Kit
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT.
Brand: Thermo Scientific™ A12900
Description
The combination of baculovirus-mediated gene delivery (BacMam) with LanthaScreen™ Cellular Assay technology enables a platform for the analysis of specific posttranslational modifications of various substrates including AKT. BacMam provides a convenient genetic delivery tool for a GFP-AKT fusion protein in the cell line of interest. This kit describes an HTS-compatible cellular immunoassay measuring phosphorylation of GFP-AKT at Thr308.
Specifications
Cell-based terbium modification activity (transient), Cell-based terbium phospho-specific activity (transient) | |
Kit contains Terbium-labeled anti-AKT antibody, BacMam AKT Virus, and 6X Lysis Buffer. Store as indicated in handling documentation. | |
TR-FRET | |
Cells not includes | |
1 kit | |
GFP (EmGFP) | |
LanthaScreen™ |
Cells Not Included | |
Fluorescence | |
Kinases, Cell Signalling | |
LanthaScreen™ | |
End Point | |
AKT1 | |
Kinases |
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For Research Use Only. Not for use in diagnostic procedures.