Invitrogen™ BacMam Histone H3K27me3 Cellular Assay Kit

BacMam Histone H3K27me3 Cellular Assay Kit

Brand:  Invitrogen™ A14159

Product Code. 11642149

Description

The BacMam Histone H3K27me3 Cellular Assay is a high-throughput screening (HTS) compatible TR-FRET assay for the interrogation of trimethylation at lysine 27 on histone H3 in a cellular format. Baculovirus-mediated gene delivery (BacMam) provides a convenient tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. Coupled with a terbium (Tb)-labeled site-specific antibody, the resulting cellular assay can be used to identify inhibitors of methyltransferases and demethylases acting on lysine 27 of histone H3.

The kit includes:

BacMam Histone H3 Reagent

LanthaScreen™ Tb-anti-histone H3K27me3 Antibody

6X LanthaScreen™ Cellular Lysis Buffer

Instrument Control Terbium TR-FRET kit

With the BacMam Histone H3K27me3 Cellular Assay Kit You Can:

Use your cell background of choice with the portability of BacMam

Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes

Conserve precious cell samples with the miniaturizable homogenous assay format

Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results
The BacMam Histone H3K27me3 Cellular Assay allows the investigation of trimethylation at lysine 27 on histone H3 with your choice of cellular backgrounds including primary cells. This enables screening for potential inhibitors of methyl transferases and demethylases associated with Lys27 trimethylation in their natural complexes in a physiologically relevant cell type.

BacMam and LanthaScreen™ Convenience Saves Sample and Time
Assays are run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as Western blotting and ELISA, making them ideal for HTS applications. By using the same BacMam reagent for GFP-Histone H3, other histone H3 modifications (Ph, Me, Ac) can be monitored just be exchanging the Terbium (Tb) labeled antibody. In addition, the application of the LanthaScreen™ technology includes all the advantages of TR-FRET detection including reduced data noise, less interference from fluorescent compounds, and high sensitivity, allowing the use of fewer cells than traditional Western or ELISA methods.

Specifications

• Content And Storage: 4°C to -20°C
• Detection Method: Fluorescent
• Includes: BacMam Histone H3 Reagent, LanthaScreen™ Tb-anti-histone H3K27me3 Antibody, 6X LanthaScreen™ Cellular Lysis Buffer, instrument control terbium TR-FRET kit
• Type: Cellular Assay Kit

• Description: BacMam Histone H3K27me3 Cellular Assay Kit, 1 Kit, Fluorescent Detection Method, Demethylases, Methyltransferases Druggable Target, LanthaScreen™ System, 4 to –20°C Storage
• For Use With (Application): Chromatin biology, pharma and biopharma, target and lead identification and validation, RNAi, epigenetics and non-coding RNA research, chromatin biology, drug discovery and development, pathway biology, cellular pathway analysis assays, BacMam-enabled cellular pathway assays, epigenetics assays for drug discovery
• Quantity: 1 Kit

For Research Use Only. Not for use in diagnostic procedures.