Invitrogen™ BRAK/CXCL14 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15454963	Invitrogen™ EHCXCL14	96 Tests
17185409	Invitrogen™ EHCXCL14X5	5 x 96 Tests
18577954	Invitrogen™ EHCXCL14X10	10 x 96 Tests

Description

The Human BRAK/CXCL14 (BRAK) ELISA quantitates Hu BRAK in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu BRAK. Principle of the method The Human BRAK solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation.

Specifications

• Accession Number: O95715
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Specificity: This ELISA pair antibody detects human CXCL14. Other species not determined.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 9547
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Inflammation
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 700 pg/mL
• Conjugate: Biotin
• Protein Family: Chemokine & Receptors
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: CXCL14
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.