IBA Lifesciences CD4 Fab-TACS™ Gravity Kit

IBA Lifesciences™ CD4 Fab-TACS™ Gravity Kit is for reversible cell isolation delivering label-free target cells.

Product Code	Brand	Quantity	Sufficient For
15869784	IBA Lifesciences 6-3202-004	4 Columns, Human	60 mL whole blood
15879784	IBA Lifesciences 6-3202-010	10 Columns, Human	150 mL Whole Blood

Description

• The kit allows the selection of CD4+ T cells directly from whole blood.
• CD4 Fab-Strep, human
• Buffer CI (10x)
• Biotin stock solution
• Fab-TACS™ Gravity Adapter
• Fab-TACS™ Flow Restrictor
• The Fab-TACS™ Gravity column is filled with a Strep-Tactin™ coated matrix made of cell-grade agarose.
• Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix.
• Subsequently, whole blood or other blood preparations pass through the column.
• Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell.
• Non-target cells are efficiently washed away.
• In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps.
• After elution, the Fab-Streps self-dissociate from the cell surfaces.
• The label-free authentic target cells are now ready for further downstream applications.

Specifications

• Description: Isolation of label-free cells
• Product Type: Non-magnetic Cell Isolation Kit
• Content And Storage: 2°C to 8°C
• Donor Source: Human Whole Blood