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Protein Purification

Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (1,609)
Magnetic Affinity Separation Particles (260)
Protease Inhibitors and Phosphatase Inhibitors (205)
Spin Columns and Gravity-flow Columns (113)
Miscellaneous Immobilization Supports (111)
Gel Filtration Media and Columns (110)
Cell Lysis Detergents (84)
In vitro Protein Expression Systems (41)
Affinity Gels (34)
Biotin-Binding Affinity Resins and Beads (34)
Miscellaneous Immobilized Affinity Ligands (26)
Cell Lysis Enzymes (18)

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1 – 15 of 1,685 results
1

Cytiva Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 10/300 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

Promotions are available
2

Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Stationary Phase Proprietary Ligand
Type Spin Column
Target Endotoxin Removal
Content And Storage Upon receipt store at 4°C.
Format Spin Column
Form Liquid
Column Type Affinity, Cellulose Resin
Formulation 22mL-capacity centrifuge columns containing 1mL resin
Capacity (Metric) Up to 2,000,000 EU/mL
Product Line Pierce™
3

Cytiva Superose™ 6 Increase Columns

High-resolution size-exclusion chromatography for studying large proteins and complexes. Cytiva Lifescience™ Superose™ 6 Increase Columns delivers high quality in size exclusion chromatography (SEC).

Promotions are available
EDGE
Particle Size 8.6μm
Ligand Type Cross-linked agarose
Content And Storage 4° to 30° in 20% ethanol
pH Range 3 to 12
Column Type Gel Filtration Column
Material Borosilicate glass
Max. Pressure 435 psi
4

Cytiva Gel Filtration Calibration Kits

Calibrate gel filtration columns simply, reliably. Cytiva Life Sciences™ Gel Filtration Calibration Kits contain a well-defined series of protein standards.

Promotions are available
Content And Storage 2° to 8°C
Product Type Calibration Kit
For Use With (Equipment) High-resolution media and prepacked column
Quantity Each
For Use With (Application) Calibration of high-resolution gel filtration media and column such as Superdex™, Superose and Sephacryl
5

Cytiva StrepTrap XT Chromatography Column

StrepTrap XP is a column packed with Strep-Tactin™ XT Sepharose resin to purify Strep-tag™ II and Twin-Strep-tag™ recombinant proteins.

Height (Metric) 25 mm
Column Type Affinity Column
For Use With (Application) Purification of recombinant proteins tagged with Strep-tag™ II and Twin-Strep-tag™
6

Thermo Scientific™ NP-40 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized and supplied as a 10% solution.

EDGE
Form Liquid
Reagent Type Detergent Solution
Product Line Surfact-Amps™
7

Cytiva HiTrap™ Con A 4B Columns

For separation and purification of glycoproteins, polysaccharides and glycolipids

Particle Size 45 to 165μm
Buffer Aqueous Buffer
Ligand Type Concanavalin A (13mg Con A/mL drained medium)
Height (Metric) 25mm
Content And Storage 4° to 8°C
pH Range 4 to 9
Product Type HiTrap Con A Sepharose 4B column
Column Type Ion Exchange Column
Material Polypropylene
For Use With (Application) Convenient separation and purification of Glycoproteins, Polysaccharides and Glycolipids
Support Type 4% Agarose
Max. Pressure 3 bar
Height (English) 0.98 in.
8

Invitrogen™ Dynabeads™ Protein A for Immunoprecipitation

Dynabeads Protein A beads are uniform, 2.8-μm superparamagnetic beads with recombinant Protein A (∼45 kDa) covalently coupled to the surface.

EDGE
Shipping Condition Ambient temperature
Content And Storage Dynabeads Protein A are supplied in PBS, pH 7.4 w/0.01% Tween-20, 0.09% NaN3 Store at 2–8°C.
Purity or Quality Grade For Research Use Only
Description Recombinant Protein A covalently bound to Dynabeads
Surface Functionality Protein A
Material Polystyrene
Concentration 30 mg/mL
For Use With (Application) Immunoprecipitation
High-throughput Compatibility High-throughput Compatible
Ligand Type Protein A
Type Superparamagnetic Beads
Target Antibodies
Format Beads in suspension
For Use With (Equipment) KingFisher™ Sample Purification System, DynaMag™ magnets
Diameter (Metric) 2.8 μm
Certifications/Compliance ISO9001 and ISO13485
Uniformity Monosized 2.8 mm (CV <5%)
Product Line Dynabeads™
9

Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

For fast and simple purification of GST-tagged proteins

Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.62 in.
Bed Volume 5 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 4 to 13
Diameter (Metric) Inner 16 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
Particle Size 45 to 165 μm
Ligand Type Glutathione
Flow Rate <20 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
10

Cytiva HisTrap™ HP Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cells. Cytiva HisTrap™ HP Prepacked Columns are prepacked with Ni Sepharose™ High Performance and designed for simple, high-resolution purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

EDGE
Flow Rate 4 mL/min. (1 mL), 5 mL/min. (5 mL)
Type Pre-packed Column
Content And Storage 4°C to 30°C
Description HisTrap HP are nickel-charged IMAC columns for high resolution his-tagged protein purification with high binding capacity for maximized recovery.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Material Polypropylene
Support Type 6% Highly Cross-linked Agarose
11

Invitrogen™ Dynabeads™ M-270 Epoxy

Dynabeads™ M-270 Epoxy beads are 2.8 μm superparamagnetic beads containing surface epoxy groups.

Promotions are available
EDGE
Shipping Condition Room Temperature
Type Epoxy Bead
Purity or Quality Grade Research Grade
Format Freeze Dried
Surface Functionality Epoxy
Sample Type Any Sample Type
Diameter (Metric) 2.8 μm
Shelf Life 24 months from date of manufacture
Elution Volume Min 5 μL
Product Line DYNAL™, Dynabeads™
12

Cytiva PD MiniTrap™ G-25 Sample Preparation Columns

Use for cleanup of biomolecules with a molecular weight >5000 Mr in sample volumes up to 0.5mL. Cytiva Life Sciences™ D MiniTrap™ G-25 Sample Preparation Columns are convenient and prepacked.

EDGE
13

Thermo Scientific™ Aprotinin Protease Inhibitor

Customize your protease inhibitor cocktail formulation with these small packages of individual protease inhibitor peptides and compounds.

EDGE
14

Invitrogen™ Dynabeads™ MyOne™ Carboxylic Acid

Surface carboxylic acid groups allow covalent amide bond formation to proteins/peptides via primary amino- or sulphydryl groups.

EDGE
Shipping Condition Room Temperature
Type Carboxylic Acid Magnetic Bead
Content And Storage Store in refrigerator (2–8°C).
Purity or Quality Grade Research Grade
Format Beads in solution
Surface Functionality Carboxylic Acid
Sample Type Any Sample Type
Diameter (Metric) 1 μm
For Use With (Application) Protein Purification and Isolation
High-throughput Compatibility High-throughput Compatible
Elution Volume Min 5 μL
Product Line Dynabeads™, MyOne™
15

Cytiva HisTrap™ FF Columns: 16 x 25mm

Use for optimized purification of histidine-tagged proteins directly from homogenized, unclarified cells by immobilized metal ion affinity chromatography (IMAC). Cytiva Life Sciences™ HisTrap™ FF Columns: 16 x 25mm is a ready-to-use column, prepacked with precharged Ni Sepharose™ 6 Fast Flow.

EDGE
Flow Rate <20 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.6 in.
Description HisTrap FF is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 3 to 12
Diameter (Metric) Inner 16 mm
Material Polypropylene
Support Type 6% Cross-linked Agarose