Thermo Scientific™ CellSensor™ CRE HEK 293T DA Assay Kit

The CellSensor™ CRE HEK 293T DA (Division Arrested) cells contains a beta-lactamase (bla) reporter gene under control of a cAMP response element (CRE) stably integrated into HEK293T cells.

Brand:  Thermo Scientific™ K1457

Product Code. 10206844

Description

The CellSensor™ CRE HEK 293T DA (Division Arrested) cells contains a beta-lactamase (bla) reporter gene under control of a cAMP response element (CRE) stably integrated into HEK293T cells. Fluorescence activated cell sorting (FACS) was used to isolate clones responsive to stimulation of the CRE pathway by forskolin. The cell line was validated for DMSO tolerance, cell number, stimulation time, substrate loading conditions, and EC50 concentration of forskolin. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can be used in other assays where a sensitive readout to intracellular changes to cAMP is needed.

Specifications

• Cell State: Division-Arrested Cells
• For Use With (Application): GPCRs, Signaling Pathway
• Product Line: CellSensor™
• Type: Division-Arrested Cells Assay Kit
• Druggable Target: Signaling Pathway

• Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
  
  Includes:
  • CellSensor™ CRE HEK293T DA cells
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  • Solution D
  • CellSensor™ CRE HEK293T CHO-K1 Assay Protocol
  • LiveBLAzer™-FRET B/G Loading Protocol
  • Certificate of Analysis
  
  The CellSensor™ CRE HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Includes: CellSensor™ CRE HEK293T DA cells, LiveBLAzer™-FRET B/G Loading Kit 70 μg, Solution D, CellSensor™ CRE HEK293T CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
• Quantity: 1 Plate
• System Type: CellSensor™

For Research Use Only. Not for use in diagnostic procedures.