Thermo Scientific Chemicals Chloroform, Molecular Biology Reagent

Description

Chloroform is a common laboratory reagent favored for use as a solvent due to the compounds relative unreactivity and miscibility with most organic compounds. Chloroform is also used in organic synthesis as a source for CCl2. In the swine tracheal smooth muscle, chloroform has been demonstrated to directly and in a concentration dependent manner induce contractions. It has been observed that Chloroform can release Ca2+ from ryanodine sensitive receptors in chloroform-induced contraction in swine tracheal smooth muscle. At concentrations of 100 and 250 ppm in mouse splenocyte, basal proliferation was increased yet a decrease in circulating neutrophils was documented.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Chloroform is a common laboratory reagent favored for use as a solvent due to the compounds relative unreactivity and miscibility with most organic compounds. Chloroform is also used in organic synthesis as a source for CCl2. In the swine tracheal smooth muscle, chloroform has been demonstrated to directly and in a concentration dependent manner induce contractions. It has been observed that Chloroform can release Ca2+ from ryanodine sensitive receptors in chloroform-induced contraction in swine tracheal smooth muscle. At concentrations of 100 and 250 ppm in mouse splenocyte, basal proliferation was increased yet a decrease in circulating neutrophils was documented.

Solubility
Soluble in water at 20°C 8 g/L.

Notes
Stabilized with approx. 50 ppm amylene

Specifications

Chemical Identifiers

• CAS: 67-66-3
• Molecular Formula: CHCl3
• Molecular Weight (g/mol): 119.37
• MDL Number: MFCD00000826
• InChI Key: HEDRZPFGACZZDS-UHFFFAOYSA-N
• Synonym: trichloromethane, formyl trichloride, methane, trichloro, trichloroform, methane trichloride, methenyl trichloride, methyl trichloride, trichlormethan, chloroforme, cloroformio
• PubChem CID: 6212
• ChEBI: CHEBI:35255
• SMILES: ClC(Cl)Cl

• Melting Point: -63°C
• Color: Colorless
• Density: 1.492 g/mL
• Boiling Point: 61°C
• Quantity: 500 mL
• Odor: Characteristic
• Physical Form: Liquid
• Refractive Index: 1.446
• UN Number: UN1888
• Beilstein: 1731042
• Merck Index: 14,2141
• Solubility Information: Soluble in water at 20°C 8g/L.
• IUPAC Name: chloroform
• Formula Weight: 119.38
• Chemical Name or Material: Chloroform, Molecular Biology Reagent

Safety and Handling

Product Identifier

• Chloroform, Molecular Biology Reagent

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Acute toxicity Category 4
• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Reproductive toxicity Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 1
• Specific target organ toxicity Category 3

Hazard Statement

• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H331-Toxic if inhaled.
• H336-May cause drowsiness or dizziness.
• H351-Suspected of causing cancer.
• H361d-Suspected of damaging the unborn child.
• H372-Causes damage to organs.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P311-Call a POISON CENTER/doctor/ .

Supplemental information

• MIXTURE LIST-Contains : chloroform, 1-pentane

RUO – Research Use Only