Invitrogen™ Corticosterone Competitive ELISA Kit

Competitive ELISA

Brand:  Invitrogen™ EIACORT

Product Code. 15805901

Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.

Description

The Corticosterone ELISA quantitates coritcosterone in serum, plasma, urine, dried fecal extracts or cell culture medium. The assay will exclusively recognize both natural and recombinant coritcosterone. Principle of the method The corticosterone solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CORTICOSTERONE is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid. [National Center for Biotechnology Information. PubChem Compound Database; CID=5753].

Specifications

• Assay Range: 78.125-10,000 pg/mL
• Description: Corticosterone Competitive ELISA Kit
• Conjugate: HRP
• Product Type: ELISA Kit
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Intraassay CV: 5.2%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Fecal Extract,50 μL; Plasma, 1 μL; Serum, 1 μL; Supernatant, 50 μL; Urine,2.5 μL
• Target: Chemical
• Total Assay Time: 1 hr. 30 min.

• Assay Sensitivity: 18.6 pg/mL
• Assay: Multiplex assay on the Luminex Platform
• Detection Method: Fluorescence
• Protein Family: Steroid hormone
• Sufficient For: 96 assays
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• Interassay CV: 7.9%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 96 Tests
• Research Discipline: Inflammation
• Storage Requirements: -20°C
• Test Time: 1 hr. 20 min.