Thermo Scientific Chemicals DL-1,4-Dithiothreitol, 99%, for biochemistry

Product Code	Brand	Quantity
10448730	Thermo Scientific Chemicals 165680010	1 g
10458730	Thermo Scientific Chemicals 165680050	5 g
10215550	Thermo Scientific Chemicals 165680250	25 g
10063023	Thermo Scientific Chemicals 165681000	100 g
15274654	Thermo Scientific Chemicals 165685000	500 g

Description

This Thermo Scientific Chemicals brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General description

• 1,4-Dithiothreitol (DTT or Cleland's reagent) reduces disulfide bonds in proteins, leading to loss of native conformation
• DTT is not stable in solution and should be prepared in small aliquots prior to use; the use of EDTA in the solution can increase the half-life of DTT
• Typically used at a final concentration of 0.1M for protein stabilization; recommended to create a 1M stock

Applications

• Protects thiol groups from oxidation and acts as a chemical chelator in solution; maintains proteins in the denatured state
• Common applications include SDS-page electrophoresis and protein isolation

Chemical Identifiers

• CAS: 3483-12-03
• InChI Key: VHJLVAABSRFDPM-IMJSIDKUSA-N
• PubChem CID: 446094
• IUPAC Name: (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol

• MDL Number: MFCD00004877
• Synonym: dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt
• ChEBI: CHEBI:42170
• SMILES: C(C(C(CS)O)O)S

Specifications

• CAS: 3483-12-03
• Melting Point: 39°C to 44°C
• Infrared Spectrum: Authentic
• Molecular Formula: C4H10O2S2
• MDL Number: MFCD00004877
• Merck Index: 15, 3419
• InChI Key: VHJLVAABSRFDPM-IMJSIDKUSA-N
• IUPAC Name: (2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
• Molecular Weight (g/mol): 154.24
• ChEBI: CHEBI:42170
• Percent Purity: ≥98.5%
• Physical Form: Powder

• Absorbance: (1 M soln. in water), (1 M soln. in water) at 0nm, 0.035 max. at 500nm
• Color: White
• Assay Percent Range: 0.99
• Linear Formula: HSCH₂CH(OH)CH(OH)CH₂SH
• Beilstein: 01, III, 2360
• Synonym: dithiothreitol, dl-1,4-dithiothreitol, dl-dithiothreitol, 1,4-dithio-dl-threitol, d-1,4-dithiothreitol, d-dtt, 2s,3s-1,4-dimercaptobutane-2,3-diol, threo-1,4-dimercapto-2,3-butanediol, 1,4-dithiothreitol, dtt
• SMILES: C(C(C(CS)O)O)S
• Transmittance: 99% min. (c=5, 660nm, water) 1cm cell
• PubChem CID: 446094
• Formula Weight: 154.24
• Grade: Biochemical
• Chemical Name or Material: DL-1, 4-Dithiothreitol

Safety and Handling

Causes serious eye irritation, Harmful if swallowed, May cause respiratory irritation, May form combustible dust concentrations in air, Causes skin irritation

Serious eye damage/eye irritation (category 2), Acute toxicity (category 4), Specific target organ toxicity after single exposure (category 3), Skin corrosion/irritation (category 2)

Product Identifier

• DL-1,4-Dithiothreitol

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H335-May cause respiratory irritation.
• H302-Harmful if swallowed.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
• P302+P352-IF ON SKIN: Wash with plenty of water/.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P332+P313-If skin irritation occurs: Get medical advice/attention.
• P337+P313-If eye irritation persists: Get medical advice/attention.

RUO – Research Use Only