Invitrogen™ Dynabeads™ SARS-CoV-2 Spike

The Dynabeads™ SARS-CoV-2 Spike consists of superparamagnetic beads coupled with a trimeric spike protein. The spike protein is a patented recombinant trimeric structure combining the S1 subunit, including the receptor binding domain (RBD), and the S2 subunit found on the surface of the SARS-CoV-2 virus.

Product Code	Brand	Volume (Metric)
17468082	Invitrogen™ 18100D	3 mL
17727443	Invitrogen™ 18100DFIVE	5 x 3 mL

Description

The Dynabeads SARS-CoV-2 Spike consists of superparamagnetic beads coupled with a trimeric spike protein. The spike protein is a patented recombinant trimeric structure combining the S1 subunit, including the receptor binding domain (RBD), and the S2 subunit found on the surface of the SARS-CoV-2 virus. The virus spike protein binds to human host cells via the angiotensin-converting enzyme 2 (ACE2) receptor to initiate virus attachment, as well as to antibodies produced in the body as a response to SARS-CoV-2 infection.

Dynabeads SARS-CoV-2 Spike features include:
• Fast SARS-CoV-2 antibody binding kinetics
• Dynabeads-facilitated automation (limited hands-on time)
• Also available in SARS-CoV-2 ELISA kits

This Dynabeads SARS-CoV-2 Spike is also included in our enzyme‐linked immunosorbent assay (ELISA) kits designed to detect and quantify the level of human SARS-CoV-2 IgG (add link to #18000D), IgM (add ink to #18010D), and Total Ig (add link to #18020D) antibodies from serum and plasma samples. These kits can be used manually with a DynaMag-96 magnet or in an automated fashion using a KingFisher instrument. The magnetic beads significantly improve the binding kinetics to cut the ELISA incubation time in half (45 minutes) compared to the more conventional plate-based ELISAs (90 minutes).

Since the spike protein binds host cells to initiate the entrance of SARS-CoV-2 virus into the epithelial cells of the lungs via ACE2, the beads may also be used for isolation of ACE2+ cells or for ACE2 protein purification.

Specifications

• Certifications/Compliance: ISO 13485
• Content And Storage: The beads are supplied in 0.05 M Tris-buffered saline (TBS) with 0.001% Tween-20 and 0.02% NaN₃.
  
  Store at 2°C to 8°C.
  
  
• Color: Brown
• Material: Magnetic Polystyrene
• For Use With (Equipment): KingFisher™ Flex or Apex Instruments, DynaMag™-96 Side Skirted, Magnetic 96-Well Separator, ELISA Plate Reader (450 nm)
• Isolation Technology: Dynabeads™ magnetic beads
• Diameter (Metric): 2.7 μm

• Concentration: 1 mg/mL
• Detection Method: Absorbance at 450 nm
• For Use With (Application): Isolation of SARS-CoV-2 Cells
• Product Type: SARS-CoV-2 Spike Protein Magnetic Beads
• High-throughput Compatibility: Yes, on KingFisher™ Flex or Apex instruments
• Shipping Condition: Wet ice (2° to 8°C)

For research use only. Not for use in diagnostic procedures.