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STEMCELL Technologies EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Immunomagnetic positive selection of human central and effector memory CD4+ T cells
Brand: STEMCELL Technologies 17865
This item is not returnable.
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Description
- This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples
- First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™
- Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™
- The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications
- Effector memory CD4+ T cells can be isolated from the CD62L- fraction
Specifications
T Cells, CD4+ | |
Cell Isolation | |
1 x 109 cells |