Thermo Scientific™ Extensor Long PCR Enzyme Mix Products

Amplify long PCR products up to 20kb with this synergistically-acting PCR enzyme blend of Taq DNA polymerase and a proprietary proofreading enzyme.

Brand:  Thermo Scientific™ AB-0794/B

Product Code. 12340130

Description

Extensor Long Range PCR Enzyme is a blend of ThermoPrime Taq DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20 kb. Extensor Long Range Enzyme includes 2 buffer formulations; Buffer 1 for templates up to 12 kb in length, and Buffer 2 for templates 12 to 20 kb and GC-rich or other difficult templates. This enzyme blend is also available in 2X Master Mix and ReddyMix formulations. The ReddyMix Master Mix contains an inert red dye and a density reagent that allows for direct loading onto agarose gels.

Extensor Long Range Enzyme Blend is an optimized mix of ThermoPrime™ DNA Polymerase and a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate long PCR products, up to 20kb. This makes the enzyme blend ideal for robust full-length amplification of long range sequences.

• Amplifies targets up to 20kb in length
• Unique enzyme blend and optimized buffer combination delivers increased accuracy and yield
• PCR products generated can be used directly in T/A cloning
• Optimized buffering systems for success with a variety of templates
• Buffer 1 for success with most templates up to 12kb in length
• Buffer 2 for templates 12-20kb and GC-rich or other difficult templates of any length

Extensor is available in a range of formats and pack sizes to suit any workflow.

Format Options:

• Enzyme Kits are the most basic format. Kits include the enzyme, PCR buffers 1 and 2, and 25mM MgCl₂ solution, each in separate vials. dNTPs must be purchased separately

• Complete PCR Kits include all the components contained in the Enzyme Kit, plus they have HPLC-purified dNTP mix. Complete PCR kits are a convenient option that provides all the components necessary for PCR

• Master Mix formats consist of a single master buffer containing all the components needed for PCR. Master Mixes are the most convenient format and significantly reduce assay set-up steps

• ReddyMix Master Mix formats consist of a single ReddyMix master buffer containing all the components needed for PCR. ReddyMix master mix can be directly loaded onto agarose gels post-PCR

• PCR Buffers: All kits are supplied with both Buffer 1 and Buffer 2 to accommodate a variety of template types. Master mixes come in either Buffer 1 or Buffer 2 formulation

Recommended for:

Amplifying fragments up to 20kb; PCR; Cloning; Long PCR

Specifications

• Content And Storage: Store at -20°C. Avoid freeze/thaw cycles
• Quantity: 400 x 25µL Rxns
• Concentration: 2X

• Description: Extensor ReddyMix Master Mix (Buffer 1 Formulation)
• For Use With (Application): Cloning

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.