Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Product Code	Brand	Quantity
10361554	Thermo Scientific™ 21328	8 x 1 mg
15925521	Thermo Scientific™ A39258	10 x 1 mg
11811205	Thermo Scientific™ 21331	100 mg

Description

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection
• Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Cleavable - disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule
• Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Medium length - spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain

Specifications

• Chemical Reactivity: Amine

For Research Use Only. Not for use in diagnostic procedures.