Thermo Scientific™ T7 RNA Polymerase (20 U/μL)

Thermo Scientific™ T7 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Product Code	Brand	Concentration	Quantity
10610301	Thermo Scientific™ EP0111	20U/µL	5,000 units
10212610	Thermo Scientific™ EP0112	20U/µL	5 x 5,000 units

Description

Thermo Scientific™ Bacteriophage T7 RNA polymerase is DNA-dependent RNA polymerase with strict specificity its respective double-stranded promoter.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.

• Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing.

Note:

Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.

Specifications

• Polymerase: T7 RNA Polymerase
• Concentration: 20U/µL

For Research Use Only. Not for use in diagnostic procedures.