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Thermo Scientific™ Uracil-DNA Glycosylase (1 U/μL)
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.
Brand: Thermo Scientific™ EN0361
29.42 GBP valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Description
Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. The enzyme shows no activity on RNA.
- Active in Fermentas buffers for restriction enzymes and thermophilic polymerases
- Source: E. coli K12 cells
- Molecular Weight: 25.6 kDa monomer
- The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests
Source:
- E.coli K12 cells
Molecular Weight:
- 25.6kDa monomer
Definition of Activity Unit:
- One unit of the enzyme catalyzes the release 1 nanomole of uracil from uracil-containing DNA template in 60 min. at 37°C
- 30mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.05% (v/v) Tween 20 and 50% (v/v) glycerol
10X Reaction Buffer:
- 200mM Tris-HCl (pH 8.2 at 25°C), 10mM EDTA, 100mM NaCl
- Inhibitors: Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
- Inactivated by heating at 95°C for 10 min. Enzyme activity is partially restored at temperatures lower than 55°C. Therefore put PCR products on ice after PCR and load directly on a gel
Recommended for:
Control of carry-over contamination in PCR (2); Glycosylase mediated single nucleotide polymorphism detection (GMPD) (3); Site-directed mutagenesis (4); As a probe for protein-DNA interaction studies (5); SNP genotyping; Cloning of PCR products (6); Generation of single strand overhangs of PCR products and cDNA
Note:
The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.UDG (UNG) is active in the presence or absence of divalent cations.
Specifications
1 U/μL | |
Uracil-DNA Glycosylase | |
200 U |
Uracil-DNA glycolase (1 U/μL) and 10X Reaction Buffer. Store at -20°C. | |
10X Reaction Buffer | |
Uracil DNA Glycosylase |
For Research Use Only. Not for use in diagnostic procedures.