Invitrogen™ FGF19 Human ELISA Kit

Sandwich ELISA Kit

Product Code	Brand	Quantity
15414993	Invitrogen™ EHFGF19	96 Tests
17844200	Invitrogen™ EHFGF19X10	10 x 96 Tests
17145439	Invitrogen™ EHFGF19X5	5 x 96 Tests

Description

The Human Fibroblast Growth Factor 19 (Hu FGF19) ELISA quantitates Hu FGF19 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF19. Principle of the method The Human FGF19 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10-FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3.

Specifications

• Accession Number: O95750
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: FGF19
• Intraassay CV: <10%
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Target: Human
• Total Assay Time: 4 hr. 45 min.

• Assay Sensitivity: 30 pg/mL
• Conjugate: Biotin
• Protein Family: Growth Factors & Receptors
• Specificity: This ELISA antibody pair detects human FGF-19. Other species not determined yet.
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 9965
• Interassay CV: <12%
• Label or Dye: HRP
• Research Discipline: Neurobiology
• Synonym: UNQ334/PRO533
• Test Time: 1 hr. 20 min.