Cytiva Ficoll PM400

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. Cytiva™ Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose, used for producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.

Product Code	Brand	Quantity
11580724	Cytiva 17-0300-10	100 g
11590724	Cytiva 17-0300-50	500 g
11570724	Cytiva 17-0300-05	5 kg

Description

• For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
• Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
• Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
• Has better osmotic properties than sucrose
• Preserves functional and morphological integrity
• Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
• Does not penetrate biological membranes
• Serves as the raw material for preparation of Ficoll-Paque gradients
• Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
• Supplied as dry powders
• Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
• Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
• Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity

Specifications

• Content And Storage: 4–30°C
• Viscosity: 5 ηr at 20°C: 10% (w/v), 20 ηr at 20°C: 20% (w/v), 60 ηr at 20°C : 30% (w/v, 180 ηr at 20°C : 40% (w/v) , 600 ηr at 20°C : 50% (w/v)
• Color: White
• Density: 1.2 g/mL

• For Use With (Application): Cell Isolation
• Endotoxin Level: 0.12 EU/mg
• Product Type: Centrifugation Media
• Form: Powder

Safety and Handling

missing translation for 'shelfLife' : 5 years