Molecular Probes™ Fluo-4 Direct™ Calcium Assay Kit

Fluo-4 Direct™ Calcium Assay Kit

Brand:  Molecular Probes™ F10471

Product Code. 10297783

Description

The Fluo-4 Direct™ Calcium Assay Kit was formulated to provide a homogeneous fluorescent calcium assay that:
1. Easily loads into cells
2. Achieves a large assay window and
3. Suppresses background fluorescence generated from the calcium indicator in complete media with little-to-no impact on the specific cellular fluorescence

This advanced formulation allows the assay to be run in a simple addition only format in the presence of serum-containing media. Fluo-4 Direct™ is the third addition to the Fluo-4 family of calcium detection reagents. Fluo-4 AM and Fluo-4 NW both require media removal before assay detection, but Fluo-4 NW adds convenience by including the PowerLoad™ reagent for ease in cell loading. The Fluo-4 Direct™ Calcium Assay Kit is similar to Fluo-4 NW in that it is formulated with PowerLoad™ for ease in cell loading, but unique in that it can be used in the presence of complete culture media and will efficiently suppress background fluorescence without sacrificing the specific cellular fluorescence generated in the assay.

Specifications

• Color: Green
• Detection Method: Fluorescence
• For Use With (Equipment): Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument, HTS Reader, Microplate Reader, Fluorescent Imager
• Dye Type: Fluorescent Dye-Based
• Form: Powder
• Product Line: Molecular Probes™
• Shipping Condition: Room Temperature

• Content And Storage:
  • 10 × 10 ml Fluo-4 Direct™ Calcium Assay Reagent (Component A)
  • 2 × 77 mg Probenecid (Component B)
  • 200 ml Fluo-4 Direct™ Calcium Assay Buffer (Component C)
  • Store at ≤-20°C. Dessicate and protect from light.
• For Use With (Application): Calcium Assay
• Product Type: Cell-based calcium flux
• Emission: Visible
• Modification: Chemical
• Quantity: 10 x 10 mL
• Sub Cellular Localization: Nucleus, Organelles, Cytoplasm

For Research Use Only. Not for use in diagnostic procedures.