GeneBLAzer™ Gα15-NFAT CHO-K1 DA Assay Kit

GeneBLAzer™ Gα15-NFAT CHO-K1 DA Assay Kit contains the promiscuous G protein.

Brand:  GeneBLAzer™ K1453

Product Code. 10792584

Description

• Gene Symbol: Gα15
• Includes: GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, GeneBLAzer™ Gα15-NFAT CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
• The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 (Cat #K1078) cell line. NFAT-bla CHO-K1 cells contain a beta-lactamase reporter gene under control of an NFAT response element stably integrated into CHO-K1 cells. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
• This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.

Specifications

• Content And Storage: Liquid Nitrogen
• For Use With (Application): GPCRs
• Type: Division-Arrested Cells Assay Kit

• Detection Method: Fluorescent
• Quantity: 1 Plate