Invitrogen™ GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit

GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit

Brand:  Invitrogen™ K1577

Product Code. 10465575

Description

The GeneBLAzer™ ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession # NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element.

Both ADRA1A CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

• Content And Storage: Liquid Nitrogen
• Detection Method: Fluorescent
• Includes: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ ADRA1A CHO-K1DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg, Solution D, GeneBLAzer™ ADRA1A CHO-K1Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
• Sample Type: CHO-K1

• Description: GeneBLAzer™ ADRA1A CHO-K1 DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, GeneBLAzer™ System, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
• Quantity: 1 Plate
• Type: Division-Arrested Cells Assay Kit

For Research Use Only. Not for use in diagnostic procedures.