Thermo Scientific™ GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 Cells

GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 cells contain the human Bradykinin Receptor B2 (B2) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line.

Brand:  Thermo Scientific™ K1790

Product Code. 10216893

Description

GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 cells contain the human Bradykinin Receptor B2 (B2) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1534) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The Bradykinin-B2-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC₅₀ concentrations of Bradykinin. In addition, GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

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Specifications

• Accession Number: NM_000623.2
• Cell State: Dividing Cells
• Description: GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 Cells, 8 x 106 Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, FRET Technique, Liquid Nitrogen Storage
• For Use With (Application): FRET
• Includes: K1790 GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 cells, GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 Assay Protocol, Certificate of Analysis
• Product Line: GeneBLAzer™
• Readout: End Point
• Sample Type: CHO-K1
• Type: CHO-K1 Cells
• System Type: GeneBLAzer™

• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.
  
  Includes:
  • GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 cells
  • GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 Assay Protocol
  • Certificate of Analysis
  
  The GeneBLAzer™ Bradykinin-B2-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Detection Method: Fluorescence
• Gene Symbol: BK2
• Population Status: Single Cell Clone Origin
• Quantity: 8 x 10⁶ Cells
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: BDKRB2
• Cell Line: CHO-K1
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.