Thermo Scientific™ GeneBLAzer™ CALCRL:RAMP3-CRE-bla FreeStyle™ 293F Cells

The GeneBLAzer™ CALCRL:RAMP3-CRE-bla FreeStyle™ 293F cells contain the human Amylin Receptor 2 (CALCRL:RAMP3), stably integrated into the CellSensor™ CRE-bla Freestyle™ 293F cell line.

Brand:  Thermo Scientific™ K1439

Product Code. 10552194

Description

The GeneBLAzer™ CALCRL:RAMP3-CRE-bla FreeStyle™ 293F cells contain the human Amylin Receptor 2 (CALCRL:RAMP3), (Accession # NM_005795.3 NM_005856.1) stably integrated into the CellSensor™ CRE-bla Freestyle™ 293F cell line. CellSensor™ CRE-bla Freestyle™ 293F cells (Cat. No. K1130) contain a beta-lactamase (bla) reporter gene under control of the cAMP response element (CRE).

CALCRL:RAMP3-CRE-bla FreeStyle™ 293F cells are functionally validated for Z'-factor and EC50 concentrations of Adrenomedullin(1-52). In addition, CALCRL:RAMP3-CRE-bla FreeStyle™ 293F cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.

Specifications

• Accession Number: NM_005855.2
• Cell State: Dividing Cells
• Description: GeneBLAzer™ CALCRL:RAMP3-CRE-bla FreeStyle™ 293F Cells, 8 x 106 Cells, ∽8,000,000 cells/vial at 8,000,000 cells/mL Quantity of Cells, FreeStyle™ 293-F Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
• Includes: GeneBLAzer™ CALCRL:RAMP3-CRE-bla FreeStyle™ 293F Cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells, GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F Assay Protocol, Certificate of Analysis
• Product Line: Freestyle™, GeneBLAzer™
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: CALCRL:RAMP3
• Cell Line: FreeStyle 293-F Cells
• Druggable Target: GPCR

• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.
  
  Includes:
  • GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells
  • GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F Assay Protocol
  • Certificate of Analysis
  
  The GeneBLAzer™ CALCRL:RAMP3-CRE-bla Freestyle™ 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• Detection Method: Fluorescence
• Gene Symbol: CALCRL:RAMP3
• Population Status: Single Cell Clone Origin
• Quantity: 8 x 10⁶ Cells
• Sample Type: FreeStyle™ 293-F
• Type: Cell Line
• System Type: GeneBLAzer™

For Research Use Only. Not for use in diagnostic procedures.