Thermo Scientific™ GeneBLAzer™ EDNRA-NFAT-bla HEK 293T Cells

GeneBLAzer™ EDNRA-NFAT-bla HEK293T cells contain the human Endothelin Type A Recpetor (EDNRA) stably integrated into the CellSensor™ NFAT-bla HEK293T cell line.

Brand:  Thermo Scientific™ K1726

Product Code. 10591814

Description

CellSensor™ NFAT-bla HEK293T cells (Cat. no. K1538) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element.

The GeneBLAzer™ EDNRA-NFAT-bla HEK293T cells are functionally validated for Z' and EC₅₀ concentrations of ET-1. In addition, GeneBLAzer™ EDNRA-NFAT-bla HEK293T cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The ENDRA receptor mediates signaling of endothelin proteins that regulate several critical biological processes, including development and function of blood vessels, production of certain hormones, and stimulation of cell growth and division. Defects in EDNRA-mediated signaling have been implicated in a wide range of diseases, including cancer, hypertension, and cardiovascular disorders.

Specifications

• Accession Number: NM_001957
• Cell State: Dividing Cells
• Detection Method: Fluorescence
• Gene Symbol: EDNRA
• Product Line: GeneBLAzer™
• Reporter Gene: BLA (Beta-Lactamase)
• Cell Line: 293T (HEK)
• Druggable Target: GPCR

• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: Contents:
  K1726 GeneBLAzer™ EDNRA-NFAT-bla HEK293T cells
  (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
  Includes:
  • GeneBLAzer™ EDNRA-NFAT-bla HEK293T cells
  • GeneBLAzer™ EDNRA-NFAT-bla HEK293T Assay Protocol
  • Certificate of Analysis
  
  Storage:
  The GeneBLAzer™ EDNRA-NFAT-bla HEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• For Use With (Application): FRET
• Population Status: Single Cell Clone Origin
• Quantity: 2 x 10⁶ Cells
• Target Entry: EDNRA
• System Type: GeneBLAzer™

For Research Use Only. Not for use in diagnostic procedures.