Invitrogen™ GeneBLAzer™ ER Alpha DA Assay Kit

GeneBLAzer™ ER Alpha DA Assay Kit

Brand:  Invitrogen™ K1393

Product Code. 10013434

Description

GeneBLAzer™ER alpha DA(Division Arrested) cells and ER alpha-UAS-bla GripTite™ cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ cell line. GeneBLAzer™UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER alpha DA cells and ER alpha-UAS-bla GripTite™cells are functionally validated for Z' and EC₅₀ concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Alternate Agonists: Betamethasone, Cortisol, Budesonide
• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER alpha GripTite™ DA cells (K1391A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Gene ID (Entrez): 2099
• Primary Agonist: Dexamethasone
• Reporter Gene: BLA (Beta-Lactamase)
• Type: Division-Arrested Cells Assay Kit
• System Type: GeneBLAzer™

• Antagonists: Milfepristone, Cortisone, Progesterone
• Cell State: Division-Arrested Cells
• Detection Method: Fluorescent
• Gene Symbol: ERα
• Product Line: GeneBLAzer™
• Target Entry: ER alpha, ESR1, ERA, NR3A1
• Cell Line: 293T (HEK)
• Druggable Target: Nuclear Receptors

For Research Use Only. Not for use in diagnostic procedures.