Thermo Scientific™ GeneBLAzer™ Gα15-NFAT CHO-K1 DA Assay Kit

The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

Brand:  Thermo Scientific™ K1453

Product Code. 10792584

Description

The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 (Cat. No. K1078) cell line. NFAT-bla CHO-K1 cells contain a beta-lactamase reporter gene under control of an NFAT response element stably integrated into CHO-K1 cells. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.

Specifications

• Cell State: Division-Arrested Cells
• Detection Method: Fluorescence
• Gene Symbol: Gα15
• Quantity: 1 Plate
• Type: Division-Arrested Cells Assay Kit
• System Type: GeneBLAzer™

• Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).
  
  Includes:
  • GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  • Solution D
  • GeneBLAzer™ Gα15-NFAT CHO-K1 Assay Protocol
  • LiveBLAzer™-FRET B/G Loading Protocol
  • Certificate of Analysis
  
  The GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• For Use With (Application): GPCRs
• Product Line: GeneBLAzer™
• Reporter Gene: BLA (Beta-Lactamase)
• Cell Line: CHO-K1
• Druggable Target: GPCR

For Research Use Only. Not for use in diagnostic procedures.