Thermo Scientific™ GeneBLAzer™ NPSR1-A CHO-K1 DA Assay Kit

The GeneBLAzer™ NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

Brand:  Thermo Scientific™ K1493

Product Code. 10033533

Description

The GeneBLAzer™ NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1129) contain a beta-lactamase (bla) reporter gene under control of the nuclear factor of activated T-cell (NFAT) response element.

The NPSR1-A CHO-K1 DA cells and NPSR1-A-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using Neuropeptide S. In addition, NPSR1-A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary found in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

Specifications

• Accession Number: NP_997055.1
• Cell State: Division-Arrested Cells
• Detection Method: Fluorescence
• Gene Symbol: NPSR1-A
• Population Status: Single Cell Clone Origin
• Quantity: 1 Plate
• Target Entry: NPSR1-A
• Cell Line: CHO-K1
• Druggable Target: GPCR

• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
  
  Includes:
  • GeneBLAzer™ NPSR1-A CHO-K1 DA cells
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  • Solution D
  • GeneBLAzer™ NPSR1-A CHO-K1 Assay Protocol
  • LiveBLAzer™-FRET B/G Loading Protocol
  • Certificate of Analysis
  
  The GeneBLAzer™ NPSR1-A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
• For Use With (Application): GPCRs
• Includes: GeneBLAzer™ NPSR1-A CHO-K1 DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70 μg, Solution D, GeneBLAzer™ NPSR1-A CHO-K1 Assay Protocol, LiveBLAzer™-FRET B/G Loading Protocol, Certificate of Analysis
• Product Line: GeneBLAzer™
• Reporter Gene: BLA (Beta-Lactamase)
• Type: Division-Arrested Cells Assay Kit
• System Type: GeneBLAzer™

For Research Use Only. Not for use in diagnostic procedures.