Thermo Scientific™ GeneBLAzer™ PR-UAS-bla HEK 293T Cells

PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

Brand:  Thermo Scientific™ K1693

Product Code. 10003443

Description

PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PR-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of R5020. In addition, PR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Alternate Agonists: GW 3965, 24 (S), 25-Epoxycholesterol, 22 (R) OH-Cholesterol, 24 (S) OH-Cholesterol
• Cell State: Dividing Cells
• Detection Method: Fluorescence, Fluorescent
• For Use With (Application): Pharma and biopharma, target and lead identification and validation
• No. of Cells: 2 x 10⁶
• Product Line: GeneBLAzer™
• Product Type: HEK 293T Cells
• Reporter Gene: BLA (Beta-Lactamase)
• Type: Cell Line

• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: PR-UAS-bla HEK 293T cells (K1103)
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen), -80°C
• Description: GeneBLAzer™ PR-UAS-bla HEK 293T Cells, 2 x 106 cells, Liquid, HEK 293T Cell Line, Human Species, Dividing Cells State, Fluorescent Detection Method, Nuclear Receptors Target, BLA (Beta-Lactamase) Reporter, Epithelial Cell Phenotype, Adherent Growth Properties
• Druggable Target: Nuclear Receptors
• Primary Agonist: TO901317
• Quantity: 2 x 106 Cells
• Sample Type: 293T (HEK)
• Target Entry: PGR, NR3C3, PR

For Research Use Only. Not for use in diagnostic procedures.