Thermo Scientific™ GeneBLAzer™ RXR alpha DA Assay Kit

GeneBLAzer™ RXR alpha DA (Division Arrested) cells and RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UASbla HEK 293T cell line.

Brand:  Thermo Scientific™ K1411

Product Code. 10358624

Description

GeneBLAzer™ RXR alpha DA (Division Arrested) cells and RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UASbla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of alpha-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RXR alpha DA cells and RXR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of 9-cis-retinoic acid. In addition, RXR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Specifications

• Alternate Agonists: Dexamethasone, Progesterone, Cortisol
• Assay Entry: Cell-based beta lactamase reporter gene
• Content And Storage: Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
  • RXR alpha HEK 293T DA cells (K1411A)
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Detection Method: Fluorescence
• Gene Symbol: RXRα
• Primary Agonist: Aldosterone
• Quantity: 1 plate
• Sample Type: 293T (HEK)
• Type: Division-Arrested Cells Assay Kit
• System Type: GeneBLAzer™

• Antagonists: Spironolactone
• Cell State: Division-Arrested Cells
• Description: GeneBLAzer™ RXR alpha DA Assay Kit, 1 Plate, ∽4,800,000 cells/vial at 4,800,000 cells/mL Quantity of Cells, 293T (HEK) Cell Line, Spironolactone Antagonists, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
• For Use With (Application): Cellular imaging, pharma and biopharma, target and lead identification and validation, target-based adme/tox assays, Cell analysis, cell-based reporter assays, cell-based and lactamase assays, drug discovery and development, nuclear receptor biology, cell-based nuclear receptor reporter assays, target-based ADME/tox assays, cellular adme assays, nuclear receptor cellular adme assays
• Includes: RXR alpha HEK 293T DA cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg
• Product Line: GeneBLAzer™
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: RXRA, RXR alpha, NR2B1
• Cell Line: 293T (HEK)
• Druggable Target: Nuclear Receptors

For Research Use Only. Not for use in diagnostic procedures.