Thermo Scientific™ GeneBLAzer™ RXR beta DA Assay Kit

Product Code. 10638723

Brand: Thermo Scientific™ K1405

Description

GeneBLAzer™RXR beta DA (Division Arrested) cells and RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

GeneBLAzer™RXR beta DA (Division Arrested) cells and RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RXR beta DA cells and RXR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of 9-cis retinoic acid (9-cis-RA). In addition, RXR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

Specifications

• Alternate Agonists: Progesterone
• Antagonists: RU 486
• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Division-Arrested Cells
• Content And Storage: Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
  • RXR beta HEK 293T DA cells (K1405A)
  • LiveBLAzer™-FRET B/G Loading Kit, 70 μg
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Description: GeneBLAzer™ RXR beta DA Assay Kit, 1Plate, ∽ 4,800,000 cells/vial Quantity of Cells, 293T (HEK) Cell Line, Fluorescent Detection Method, Nuclear receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Adherent Growth Properties, –80°C/Liquid Nitrogen Storage
• Detection Method: Fluorescence
• For Use With (Application): Cellular imaging, pharma and biopharma, target and lead identification and validation, target-based adme/tox assays, Cell analysis, cell-based reporter assays, cell-based and lactamase assays, drug discovery and development, nuclear receptor biology, cell-based nuclear receptor reporter assays, target-based ADME/tox assays, cellular adme assays, nuclear receptor cellular adme assays
• Gene Symbol: RXRβ
• Includes: RXR beta HEK 293T DA cells (K1405A) LiveBLAzer™-FRET B/G Loading Kit, 70μg
• Primary Agonist: R5020
• Product Line: GeneBLAzer
• Quantity: 1 Plate
• Reporter Gene: BLA (Beta-Lactamase)
• Sample Type: 293T (HEK)
• Target Entry: RXR beta, NR2B2, RXRB
• Type: Division-Arrested Cells Assay Kit
• Cell Line: 293T (HEK)
• System Type: GeneBLAzer™
• Druggable Target: Nuclear Receptors

For Research Use Only. Not for use in diagnostic procedures.