Gibco™ Advanced MEM

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Brand:  Gibco™ 12492021

Product Code. 11540436

Description

Advanced MEM (Minimum Essential medium) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic MEM, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.ⁱ Cells successfully cultured in Advanced DMEM / F-12, with no adaptation, include PK-15, MDCK, Vero, WI-38 and Hep-2.

This Advanced MEM is manufactured as follows:

With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ Advanced MEM is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced MEM requires supplementation with 1-5% Fetal Bovine Serum and 4mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

ⁱPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.

ⁱPaul Price, Brad Stiles, David Staines, Ethel Evege, Femi Fatunmbi, Kate Wagner, Lori Nestler, David Jayme. Advanced Media as Alternatives to Classical Basal Media. FOCUS, 2003. 25.2:3-6.

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco Advanced MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Classification: Animal Origin
• Form: Liquid
• Serum Level: Reduced Serum
• Sterilization Method: Sterile-filtered
• Without Additives: No Glutamine, No HEPES
• Product Line: Gibco™
• Shipping Condition: Ambient

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Concentration: 1 X
• Product Type: MEM (Minimum Essential Medium)
• Sterility: Sterile-filtered
• With Additives: Low Glucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Quantity: 10 x 500 mL

For Research Use Only. Not for use in diagnostic procedures.