Cytiva Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Brand:  Cytiva 17-5132-03

Product Code. 11505025

Description

• Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
• Protein binding capacity: ≈10mg recombinant GST/mL media (flowrate dependent)
• Binding capacity and high flow properties allow scale-up
• Mild elution conditions preserve protein antigenicity and function
• Stable in all commonly used aqueous buffers
• pH stability: 3 to 12

Specifications

• Binding Capacity: >10 mg/mL
• Flow Rate: <450 cm/hr.
• Matrix: Highly cross-linked 4% agarose
• Support Type: 4% Highly Cross-linked Agarose
• Max. Operating Pressure: base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
• Quantity: 500 mL
• Ligand Type: Glutathione and 10-carbon linker arm

• Description: Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
• Kit Contents: 10 Carbon Spacer Arm, 4% Agarose, 90 μm Particle Size
• pH Range: 3 to 12
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Particle Size: 90 μm
• Type: Chromatography Media

Safety and Handling

Product Identifier

• Glutathione Sepharose 4 Fast Flow

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol