Thermo Scientific™ UltraLink™ Hydrazide Resin

Immobilize polyclonal antibodies or other glycoproteins, via periodate-oxidized sugars, onto hydrazide-activated polyacrylamide beads for AP, IP or pull-down assays.

Brand:  Thermo Scientific™ 53149

Product Code. 11809410

Description

Thermo Scientific™ GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions.

The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions. Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns. Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability. Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity.

Highlights:

• High-capacity – immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin

• Fast and efficient – immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation)

• Specific immobilization – the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate

• Stable attachment – resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required

• Preserves antibody function – immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target

• Convenient sizes – choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns

UltraLink Hydrazide Resin:

• Ligand: hydrazide
• Support: Thermo Scientific UltraLink Biosupport
• Spacer arm: 22 atoms
• Loading: activated with at least 15μmol hydrazide per milliliter of settled resin
• Format: 50% resin slurry in 0.02% sodium azide

Includes:

Kits contain GlycoLink Columns; coupling buffer, coupling catalyst and wash buffer; oxidizing reagent; desalting columns, PBS buffer pack; and column accessories

Recommended for:

Purify antigens using covalently coupled antibodies; Determine binding partners for a glycoprotein of interest; Perform large-scale affinity purifications

Specifications

• Capacity (Metric): 1 to 10 mg Oxidized Antibody or Glycoprotein Immobilizing per mL Resin
• Content And Storage: Store at 4°C.
• Quantity: 10 mL
• Stationary Phase: Hydrazide
• Column Type: Affinity, UltraLink™ Resin

• Description: UltraLink Hydrazide Resin
• Product Line: UltraLink™
• Type: Resin
• Format: Bottle

For Research Use Only. Not for use in diagnostic procedures.