Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

For fast and simple purification of GST-tagged proteins

Product Code	Brand	Quantity
11575125	Cytiva 28-4017-48	5 x 5 mL
11743319	Cytiva 28-4017-47	1 x 5 mL

Description

• Prepacked with Glutathione Sepharose™ 4B media, featuring a glutathione ligand coupled to 4% agarose
• May be used with syringe, peristaltic pump or ¨A¨KTA design chromatography system
• Purifies GST-tagged proteins from bacterial lysates and other glutathione S-transferases or glutathione-dependent proteins
• Bed dimensions: 16 x 25mm
• Flow velocity: <20mL/min.
• Average particle size: 90μm

Media

• Matrix: Highly cross-linked 4% agarose
• Binding capacity: >25mg horse liver GST/mL
• pH stability working range: 4 to 13

Specifications

• Bed Volume: 5 mL
• Diameter (Metric) Inner: 16 mm
• Flow Rate: <20 mL/min.
• pH Range: 4 to 13
• Content And Storage: 4°C to 30°C, 20% Ethanol
• Particle Size: 45 to 165 μm
• Type: Pre-packed Column

• Diameter (English) Inner: 0.62 in.
• Description: GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
• Material: Polypropylene
• Support Type: 4% Highly Cross-linked Agarose
• Max. Operating Pressure: 5 bar (0.5 MPa, 70 psi)
• For Use With (Application): GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
• Ligand Type: Glutathione

Safety and Handling

Product Identifier

• GSTrap 4B 5 ml, 5 x 5 ml

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P235-Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol