Thermo Scientific™ Halt™ Protease Inhibitor Cocktail (100X)

Inhibit proteolytic degradation during cell lysis and protein extraction with these ready-to-use concentrated stock solutions of protease inhibitors.

Product Code	Brand	Description	Quantity
11834101	Thermo Scientific™ 78429		5 mL
10720825	Thermo Scientific™ 78430		24 x 100 μL
11814111	Thermo Scientific™ 78438		10 mL
10516495	Thermo Scientific™ 87786		1 mL

Description

Thermo Scientific™ Halt Protease Inhibitor Cocktails are ready-to-use concentrated stock solutions of protease inhibitors for addition to samples to prevent proteolytic degradation during cell lysis and protein extraction procedures.

Halt Protease Inhibitor Cocktails are 100X solutions containing optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide (DMSO). The cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. The identity and concentration of each component is fully disclosed, leaving no uncertainties about variables and compatibility with experiments. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively. The stable, concentrated liquid cocktails are easy to use, and the several different package sizes make Halt Protease Inhibitor Cocktails exceptionally convenient for either small-scale or large-scale uses.

Highlights:

• Better than tablets – the refrigerator-stable, 100X liquid format is more effective and easier to use than individual inhibitors or other formats; just pipette exactly the amount you need

• No proprietary ingredients – fully disclosed formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin and pepstatin A in DMSO

• Multiple package sizes – choose among several package sizes, including single-use microtubes that keep protease inhibitors fresh and reduce the risk of reagent contamination

• Two popular formulations – regular formulation includes a separate vial of EDTA solution for optional metalloprotease inhibition; EDTA-free formulation ensures compatibility with isoelectric focusing

• Lysis buffer compatible – use with Thermo Scientific Pierce™ Cell Lysis Buffers or nearly any other commercial or homemade detergent-based lysis reagent

Includes:

Protease inhibitor cocktails with EDTA include separate 0.5M EDTA solution.

Specifications

• Product Type: Protease Inhibitor Cocktail
• Form: Liquid
• Buffer: Lysis Buffer
• Inhibitors: Protease Inhibitor

• Content And Storage: Upon receipt store at 4°C
• Product Line: Halt™
• Reagent Type: Protease Inhibitor

Safety and Handling

Product Identifier

• Halt Protease Inhibitor Cocktail (100X)

Signal Word

• Warning

Hazard Category

• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2

Hazard Statement

• H315-Causes skin irritation.
• H319-Causes serious eye irritation.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P264-Wash thoroughly after handling.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : Benzenesulfonylfluoride, 4-(2-aminoethyl)-, hydrochloride (1:1)

For Research Use Only. Not for use in diagnostic procedures.