Gibco™ Ham's F-10 Nutrient Mix

Ham's F-10 Nutrient Mix

Brand:  Gibco™ 31550023

Product Code. 11564436

Description

Ham's F-10 Nutrient Mixture (F-10) was designed for serum-free growth of Chinese Hamster Ovary (CHO) cells. F-10 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including COS-7, primary rat astrocytes, and rat prostate epithelial cells.

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12).

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ F-10 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer a comparable Gibco™ F-10 product made in our Grand Island facility. This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Gibco™ F-10 contains no proteins or growth factors. Therefore, F-10 often requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

• Certifications/Compliance: ISO 13485 standards, FDA
• Description: Ham's F-10 Nutrient Mix, 500mL, 1 X Concentration, Liquid, 7.0 to 7.4 pH, 12 Months Shelf Life, 275 to 315 mOsm/kg Osmolality, With Sodium Pyruvate, L-glutamine, Phenol Red, 2 to 8°C Storage, For Mammalian Cell Culture
• Concentration: 1 X
• Product Type: Ham's F-10 Nutrient Mix
• Without Additives: No HEPES
• pH: 7.0 to 7.4

• Content And Storage: 2 to 8°C
• Cell Line: CHO
• Form: Liquid
• With Additives: Glutamine, Phenol Red, Sodium Pyruvate
• For Use With (Application): Mammalian cell culture
• Quantity: 500 mL