Thermo Scientific™ High Select™ Depletion Spin Columns

Decrease albumin, immunoglobulins, and other high-abundance proteins from human serum or plasma in preparation for mass spectrometry analysis, or 1D and 2D gel electrophoresis using High Select depletion spin columns or resin.

Product Code	Brand	Description
15802684	Thermo Scientific™ A36365	HSA/Immunoglobulin Depletion Mini Spin Columns
15812684	Thermo Scientific™ A36366	HSA/Immunoglobulin Depletion Mini Spin Columns
15822684	Thermo Scientific™ A36367	HSA/Immunoglobulin Depletion Midi Spin Columns
15832684	Thermo Scientific™ A36368	HSA/Immunoglobulin Depletion Resin
15862684	Thermo Scientific™ A36371	Top14 Abundant Protein Depletion Midi Spin Columns
15870863	Thermo Scientific™ A36372	Top14 Abundant Protein Depletion Resin
15842684	Thermo Scientific™ A36369	Top14 Abundant Protein Depletion Mini Spin Columns
15852684	Thermo Scientific™ A36370	Top14 Abundant Protein Depletion Mini Spin Columns

Description

Analysis of human fluids is often complicated by the presence of high concentrations of serum proteins, including albumin and IgG that can make up more than 70% of total serum protein. Removal of albumin, immunoglobulins, and other abundant proteins is essential for the study of low-abundance proteins. Thermo Scientific High Select HSA/Immunoglobulin and Top 14 Abundant Protein depletion spin columns and resins enable depletion of the most abundant proteins in human serum and plasma for improved detection of low-abundance proteins using mass spectrometry analysis or 1D or 2D gel electrophoresis.

• Flexible—resin provided in mini and midi column formats as well as bulk for custom filling options
• Optimized—removes >95% of IgG and >95% of albumin (HSA/Immunoglobulin); removes >95% of IgG and >95% of albumin, plus 12 other abundant proteins (Top 14)
• Fast—process samples in ∼10 minutes
• Economical—cost-effective spin columns are priced for single use
• Reduced carry-over—one-time use prevents protein carryover and experimental variability

High Select HSA/Immunoglobulin Depletion Resin uses highly specific, immobilized anti-HSA and anti-immuoglobulin antibodies (IgG, IgA, IgM, IgD, and IgE) to remove human serum albumin (HSA) and all major subclasses of immunoglobulins from serum and plasma. High Select Top14 Abundant Protein Depletion Resin uses highly specific immobilized antibodies to remove human serum albumin (HSA), albumin, IgG, IgA, IgM, IgD, IgE, kappa and lambda light chains, alpha-1-acidglycoprotein, alpha-1-antitrypsin, alpha-2-macroglobulin, apolipoprotein A1, fibrinogen, haptoglobin, and transferrin from serum, plasma, or spinal fluids. The resins are provided in an economical and convenient spin column format specifically designed for one-step processing and single use or bulk format for custom filling.

Specifications

• Product Line: High Select™
• Label or Dye: Unlabeled
• Starting Material: Plasma, Serum
• Workflow Step: Protein Depletion

• Content And Storage: Store at 2–8°C.
• Shipping Condition: Wet Ice
• Final Product Type: Protein