Cytiva HiTrap™ IMAC Columns

For optimizing purification of histidine-tagged proteins when Ni ⁺² is not the best choice of metal ion

Brand:  Cytiva 17-0921-02

Product Code. 10697275

Includes: Instructions, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

Description

• Conveniently charge with your metal of choice
• Can be used with a syringe, peristaltic pump, or liquid chromatography system
• Available prepacked with IMAC Sepharose™ High Performance (HP), with small bead size for high performance purification, or IMAC Sepharose 6 Fast Flow (FF), with larger bead size for easy scaleup
• High binding capacity: ≥40mg histidine-taged protein/mL medium (protein/metal ion dependent)
• Stable in the presence of all commonly used buffers
• Available in 1mL and 5mL sizes

Specifications

• Bed Volume: 1 mL
• Flow Rate: <4 mL/min.
• Kit Contents: Prepacked with IMAC Sepharose 6 Fast flow media
• Material: Polypropylene
• pH Range: 3 to 12
• Product Type: HiTrap IMAC Fast Flow Column
• Quantity: 5 x 1 mL

• Diameter (English) Inner: 0.27 in.
• Diameter (Metric) Inner: 7 mm
• For Use With (Equipment): Syringe, peristaltic pump or liquid chromato graphy system
• Max. Pressure: 42 psi
• Particle Size: 90 μm
• Content And Storage: 4°C to 30°C
• For Use With (Application): For optimizing purification of His-tagged Proteins

Safety and Handling

Product Identifier

• hitrap IMAC FF

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3

Hazard Statement

• H226-Flammable liquid and vapour.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P235-Keep cool.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : ethanol