Thermo Scientific™ Hypersil GOLD™ PREP C4 HPLC Columns

Use for hydrophobic analytes that bind too tightly on other reversed-phase columns.

Product Code	Brand	Length (Metric)	Diameter (Metric)
16358576	Thermo Scientific™ 25505-109270A	100 mm	20 mm
16368576	Thermo Scientific™ 25505-109270XL	100 mm	25 mm
16378576	Thermo Scientific™ 25505-259270A	250 mm	20 mm
16388576	Thermo Scientific™ 25505-259270XL	250 mm	25 mm

Description

Attain faster separations for hydrophobic analytes when you scale up using Thermo Scientific™ Hypersil GOLD™ C4 reversed phase preparative HPLC Columns, for your purification needs. These C4 columns provide excellent sensitivity and high efficiency, highlighting a lower hydrophobicity than C8 or C18 columns. Our columns come in a variety of dimensions and an option for XtendedLife hardware which improves column lifetime by maintaining a uniform packed bed.

Similar selectivity, less retention than C18 or C8 columns

• Outstanding retention and separation of very hydrophobic analytes with this short alkyl chain length, low hydrophobicity column.
• Best for analysis of hydrophobic polypeptides and proteins.
• C4 (USP L26) selectivity.

Preparative HPLC Column Hardware Options - based on column inner diameter (i.d.) and column performance needs.

Standard Threaded and Standard Flange Hardware

• Designed for standard applications, expertly packed and robustly designed to provide versatile products for routine use.
• Standard threaded hardware for column i.d. < 40mm.
• Standard flange hardware for column i.d. ≥ 40mm.
• Standard hardware can be modified on request to be compatible with SFC.

XtendedLife Hardware

• Improves column lifetime by incorporating an internal Dynamic Axial Compression (DAC) system, ensuring the media bed is continuously packed tightly.
• Compatible with SFC and is available in dimensions up to 50mm i.d.

Customer Specific Orders

• If there is a Thermo Scientific stationary phase you are currently using and would like to obtain in a preparative scale not shown or would like to discuss a specific column dimension, we would be happy to discuss your requirements.
• Please contact your local sales representative for Customer Specific Orders.
• Custom built columns are not eligible for returns.

About the Hypersil GOLD family of HPLC columns

Meet the challenges of modern chromatography with state-of-the-art columns that provide outstanding performance and consistent separations. Twelve different chemistries are available so you can optimize separations and maximize productivity. Get outstanding peak shapes in reversed phase, ion exchange, HILIC, or normal phase chromatography.

Specifications

• Particle Size: 5 μm
• Pore Size: 175 Å
• Temperature: 60°C
• Stationary Phase: C4
• Carbon Load: 5%
• Endcapped: Yes

• pH: 2 to 8
• Surface Area: 220 m²/g
• USP Type: L26
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Column Type: Reversed Phase
• Phase: Reversed Phase