Invitrogen™ IL1RL1/ST2 Human ELISA Kit

Sandwich ELISA Kit

Brand:  Invitrogen™ EHIAST2X5

Product Code. 17195459

Includes: Pre-coated plate(s), standard, peroxidase conjugated detector antibody, assay buffer, wash buffer, chromogen, stop solution, plate covers and lot-specific technical data sheet.

Description

The Human IL1RL1/ST2 ELISA quantitates Hu IL1RL1 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL1RL1. Principle of the method The Human IL1RL1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

ST2 is a member of the interleukin 1 receptor family. Studies of ST2 in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. ST2 belongs to the interleukin-1 receptor family with three Ig-like C2-type (immunoglobulin-like) domains and a TIR domain. ST2 is a receptor for interleukin-33 (IL-33) which upon stimulation recruits the signaling molecules MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Reports also suggest its possible role in helper T-cell function as well as in cell growth. Three isoforms of ST2 have been reported where isoform C is a membrane form and isoform B is a secreted form. ST2 may be induced by proinflammatory stimuli, and may also be found in serum of autoimmune disease. Different isoforms are restricted to different tissues while high expression of this protein is evident in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine.

Specifications

• Accession Number: Q01638
• Assay Sensitivity: 30.5 pg/mL
• Assay: Multiplex assay on the Luminex Platform
• Detection Method: Fluorescence
• Protein Family: Cytokine
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• Gene ID (Entrez): 9173
• Interassay CV: 8.5%
• Kit Contents: Chromogen, SAV-HRP, Pre-coated 96 well plates, Adhesive Plate Covers, Wash Buffer, Biotinylated Detection Antibody, Standard, Stop Solution, Assay Diluent Concentrate
• Quantity: 5 x 96 Tests
• Research Discipline: Inflammation
• Storage Requirements: -20°C
• Test Time: 1 hr. 20 min.

• Assay Range: 31.25 pg/mL to 2000 pg/mL
• Description: Human Growth Stimulation-Expressed Protein ST2 ELISA Kit
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: IL1RL1
• Intraassay CV: 4.3%
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma, 12.5 μ;L; Serum, 12.5 μ;L; Supernatant, 50 μ;L
• Target: Human
• Total Assay Time: 3 hr.