Cytiva RNAspin Mini Isolation Kit

Extract high-quality RNA from a wide range of samples rapidly. Cytiva Life Sciences™illustra™ RNAspin Mini Isolation Kit can be used to isolate total RNA from cultured cells, tissue, bacteria, or yeast.

Brand:  Cytiva 25-0500-70

Product Code. 11535065

Includes: Protocol booklet with detachable, quick reference card, necessary components for specified number of preps, including prefilters and DNase I

Description

• Recovers total RNA due to removal of genomic DNA through on-column DNase I treatment
• Can be used on cultured cells, tissue, yeast and hard-to-lyse bacterial strains
• Prefilters reduce lysate viscosity for maximum yield and purity
• Results can be obtained with small amounts of sample
• Binding capacity: 100μg
• Sample size: 30mg (animal tissue), up to 5 × 10⁶ cells (cell culture)
• Elution volume: 40 to 120μL
• RNA Purity (A260/A280): 1.8 to 2.2
• Total time of preparation: <30 min/6 preps

Quantitative reverse transcriptase PCR (QRT-PCR), Northern blot, and microarray experiments

Specifications

• Description: illustra RNAspin Mini RNA Isolation Kit is designed for rapid extraction of high-quality RNA from a wide range of sample types.
• Includes: Protocol booklet with detachable, quick-reference guide and necessary components, including prefilters and DNase I
• Product Type: illustra RNAspin Mini Kit
• Sample Volume: <30 mg
• Yield: Up to 100μg

• For Use With (Application): Used for the isolation of high-quality total RNA from a wide range of source material including hard-to-lyse bacterial strains. Purified RNA is suitable for use in sensitive downstream applications such as quantitative reverse transcriptase PCR (qRT-PCR),
• No. of Reactions: 20 Preps
• RNase: 1.8-2.2
• Test Time: < 30 min/6 preps

Safety and Handling

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H302 + H312 + H332-Harmful if swallowed, in contact with skin or if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P273-Avoid release to the environment.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P312-Call a POISON CENTER/doctor/if you feel unwell.
• P501-Dispose of contents/container to .